For each patient 5- times diluted serum was used in two different arrays for the detection of bound C3-IgM and IgG-C4.
Dried arrays were rinsed in PBS for 15 minutes before use, then incubated with diluted serum at 37☌ for 1 hour, providing suitable conditions for complement activation. Features were printed in triplicates of 1:5 serial dilutions then stored at 4☌ in sealed bags. Serum sample was centrifuged for 6min at 15000g for removing particlesĪntigens were spotted onto nitrocellulose-covered FAST slides (GE Healthcare) using BioOdyssey Calligrapher miniarrayer (BioRad). GEO help: Mouse over screen elements for information.
